New 3-HRP conjugate

Advancing Biochemical Analysis: Introducing the Advanced eQuant-HRP

Optimized Ratio of HRP to Biotin-Binding Protein

conjugate over current market offerings. Firstly, it uniquely enables direct quantification of biotinylated elements. Secondly, we have engineered eQuant-HRP to achieve an optimal balance between Horseradish Peroxidase (HRP) and biotin-binding protein. Thirdly, this carefully calibrated balance significantly enhances specificity in various assays, leading to more accurate and reliable detection.

Enhanced Efficiency and Direct Quantification

Our design of eQuant-HRP enables more efficient binding to biotinylated targets. This conjugate’s ability for direct quantification ensures straightforward and less time-consuming detection and measurement of biotinylated elements, especially beneficial in high-throughput settings where speed and accuracy are critical.

Standard HRP conjugate

Advanced 3-HRP conjugate for a direct quantification

Significant Reduction in Non-Specific Binding

The improved HRP to biotin-binding protein ratio in eQuant-HRP significantly reduces non-specific binding. This reduction not only increases the accuracy of assay results but also minimizes potential interference, providing clearer and more definitive outcomes.

Conclusion: Advancements in Biotin-Binding Protein Technology

In summary, the introduction of eQuant-HRP marks a significant advancement in biotin-binding protein technology. Its optimized ratio of HRP to biotin-binding protein, leading to decreased non-specific binding, and its capability for direct quantification, make it a superior choice for biochemistry professionals.


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High-Quality Enzyme Sourcing and Development

To create such a conjugate, e-proteins insists on receiving the highest quality enzyme from its suppliers, one that is unavailable in the global market. Major suppliers struggle to provide HRP with the required quality for the perfect 3 HRP/1 binding protein mix. Therefore, e-Proteins has collaborated with its supplier to test several purification methods to achieve the necessary quality level.

Advancements in Purification and Production

During four years of R&D, we actively developed and implemented a new three-step purification protocol. Firstly, this protocol enables us to extract and purify the enzyme directly from the plant. Subsequently, our next objective is to boost production yield. Consequently, this increase will ensure that all our customers can transition to this new conjugate without facing significant additional costs.

CLIA Application

HRP finds use in ChemiLuminescent ImmunoAssays (CLIA). This technology merges chemiluminescence’s sensitivity with immunoreaction specificity. HRP transforms a substrate into a chemiluminescent signal, emitting photons in the visible or near-visible range. The light produced directly correlates with the target analyte’s quantity in the sample. The homogeneity of 3HRP enables direct quantification.

CLIA holds a significant advantage over the Elisa Method. It is far more sensitive due to signal amplification and multiplication. Moreover, it operates without the need for incubation time or stopping reagents.

All About

Horseradish peroxidase, a vital enzyme from plant sources, consistently draws interest from researchers across various disciplines due to its practical and commercial uses. Researchers have made advancements in understanding its structure and catalytic mechanism through protein engineering and other techniques. Currently, they are investigating the enzyme’s physiological role, informed by new insights into the plant peroxidase gene family of Arabidopsis thaliana.

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