Why Neutralite Avidin Presents a Lower Non-specific Binding

This derivative is relatively free of non-specific binding to cells and DNA due to its lower isoelectric point (pI=6). At this value of pI=6; Lysines remain available. e-proteins can offer custom derivatives with a lower (pI=5,2). The only consequence of this change is the limitations for the derivation and conjugation due to the lack of lysine.

This picture shows the sequence’s differences between Neutralite Avidin and Streptavidin and it justifies why Neutralite Avidin is better than Streptavidin.

How Neutralite Avidin is a True Replacement for Streptavidin with high Signal/Noise

Neutralite Avidin which has high signal/noise (S/N), is carbohydrate-free and neutral, with the additional benefit of a large number of amino acids available for derivatization and the absence of Arg-Tyr-Asp (RYD) sequences. Altogether, these molecular properties result in the lowest non-specific binding among known biotin-binding proteins such as streptavidin. Its specific activity is close to the theoretical maximum of around 16 µ/mg of protein. Let’s learn more about this novel protein. It is a good replacement for Neutravidin with a comparatively good specific activity.

Neutralite Avidin Properties

This innovative protein derivative is a form of lite-avidin with a neutral charge. This neutralization reduces non-specific binding by minimizing the interaction between the protein and charged surfaces or materials. This can be useful in some applications where non-specific binding or high signal/noise is a concern, such as in immunoassays or other biochemical assays where Strepavidin is used.

• This derivative is a deglycosylated and isoelectrically neutral form of Avidin.
• It can replace streptavidin with an advantage because its lower extent of non-specific binding reduces background signals.
• Its structure consists of four identical subunits, each containing a binding site for biotin similar to Avidin.
• Due to its acidic pI, the Neutralize Avidin is relatively free from non-specific binding to cells and DNA.
• Its near-neutral pI (6.3) offers the great advantage of minimizing non-specific adsorption
• As its lysine residues remain available for derivatization or conjugation, Neutralite Avidin can also be conjugated to
  • an enzyme (i.e., horseradish peroxidase)
  • a fluorescent dye to visualize biotinylated proteins on membranes

This performance of Neutralite Avidin is higher than the Neutravidin, indeed the e-Proteins product shows a higher specific activity and no solubility issue at the pI.

Streptavidin vs Neutralite Avidin

This avidin derivative is a good replacement for Streptavidin with high signal/noise. The product offers solutions to Streptavidin users who are facing issues with their applications:

• No non-specific binding to cell-surface proteins (better than Streptavidin)
• High signal-to-noise ratio in detection systems (better than Streptavidin)
• Highest specificity for biotin binding

Neutralite Avidin Application

This derivative has many applications and some of the most important ones such as:

• Western/Southern/Northern Blotting
• ELISA
• Immunohistochemical staining
• Fluorescence activated cell sorting

Evolution of the Neutralite Avidin Development

In 2012, a new R&D program started to improve product quality to reach higher performance in terms of specific activity, deglycosylated level, isoelectric point, and reproducibility. In 2018, this program successfully ended with NAX (Ultra Neutralite Avidin) with higher performance. Its sales to our direct end-customers started directly.

e-Proteins has launched an R&D project to master the Neutralite Avidin polymerization and better respond to its customers’ expectations of low and high polymerized lots. After 5 years of research, our R&D team has implemented a new approach that avoids the natural olimegorisation of the product. On the other hand, our engineering department has developed a controlled polymerisation process that guarantees the production of Poly-Lite-avidin.

For the Neutralite Avidin, e-proteins concentrate their efforts on the deglycolisation level in order to reduce non-specific binding and protein activity as much as possible. As a result, our product has an exceptional specific activity > 15.5 U/mg and successfully competes with steptavidin in many applications with a deglycolisation level of around 95%.

Contact Us if you want to learn more about our product or have any queries. Our company guarantees the best prices for the highest quality.