How Ultracoat70 Ensures A Higher Binding Signal for Detection

This innovative product presents a higher binding signal due to its multi-layers forms. The higher binding capacity improves the test sensibility for accurate detection. It is not only the best alternative to cross-linking streptavidin technology or “polystreptavidin R” but also has improved properties. Let’s learn the distinct characteristics of Ultracoat70.

Ultracoat70 from e-Proteins boosts signal intensity by 173%. Today this derivative presents the higher binding signal for detection on the market.

Ultracoat70 Characteristics

• It is homogeneous with a constant extinction coefficient and molecular weight < 500 kDa as compared to the conventional polystreptavidin R.
• The product has only covalent binding between the molecule without linker.
• Its conformation stays simple because of covalently linked molecules.
• Temperature, pH, protein concentration, ionic strength, as well as molecular crowding have a limited impact on the olimegorized protein.
• The specific polymerization process enables the use of Lysine (available for further conjugation and derivatization).
• e-Proteins product UltraCoat70 can be used in any range of pH.
• Ultracoat70 is a lyophilised powder which can be reconstitute with any buffer. The 0,22 micron filtration is allowed.
• Have a higher level of dimers, trimers and quadrimer comparing the cross-linking steptavidin profile.

Thanks to a multi-layered design, maximizing bonds per surface area

e-Proteins developed two chemical modifications to keep the product soluble for use in acid/neutral reconstitution buffer as well as neutral/basic reconstitution buffer, a novel approach to high-performance in vitro diagnostics (IVDs) and direct substitution. This meets the specific needs of the customer regarding the reconstitution buffer.

Ultracoat70 Development Background

In 2012, Customers who use the cross-linking streptavidin technology requested e-Proteins to develop a new product that merges the benefits of Neutralite Avidin with the high sensitivity of cross-linking streptavidin. In one word, the customer looks for a new derivative that offer a higher binding signal for detection.

After eight years of research, e-Proteins developed a process to produce Poly-Neutralite Avidin with a profile that has more dimers, trimers, and quadrimers than cross-linking PolySteptavidin R.

Neutralite Avidin, with a nearly neutral charge, no oligomannosidic glycan as well as no Arg-Tyr-Asp (RYD) amino acids sequence, has reduced background or non-specific bio-recognition by 75% for years.

If you are interested in our product and have any questions, please do not hesitate to Get in Touch with us. At e-Proteins, you can buy with confidence, knowing that you will get the lowest prices on the best products.