Ultracoat50

Ultracoat50: Enables a Higher Binding Performance for Purification

The e-proteins derivative for purifcation is superior to Streptavidin not only in terms of higher binding performance for improving purification yield but also has price competitiveness.

This derivatives process is based on the reliable Poly-Lite Avidin process with different working points that generate optimized polymerization and less waste that directly impacts the final price.

Ultracoat50 provides at least 25% more binding signals when coated on beads than Streptavidin, and biochips plastic membranes demonstrate greater target capture efficiency when coated on magnetic particles or magnetic nanoparticles.

Ultracoat50 with 2 binding layers

This pic shows how Ultracoat50 presents an increased biotin binding capacity compared to Streptavidin.

Ultracoat50 Properties

  • Binds biotinylated ligands such as proteins, nucleic acids, and peptides.
  • Fast reaction kinetics.
  • Covalently bonded Lite Avidin Coating.
  • Lower non-specific binding compared to Streptavidin.
  • Highest throughput and precision.
  • High solubility.
  • No aggregation due to linkers.
  • Constant extinction coefficient.
  • Homogenous with dimers, enabling a double binding layer with a limited spheric encumbrance (low molecular weight < 250 kDa).

Variations in Ultracoat50

  • High-speed version
  • Biotin binding ranges: from 2500 to 7500 pmol/mg
  • 2 versions for 2 different pH range usage according to the customer protocol (cover full pH range).

FAQs

What are magnetic beads for purification ?

Magnetic beads are composed of tiny (20 to 30 nm) iron oxide particles, such as magnetite (Fe3O4), which give them superparamagnetic properties. Superparamagnetic beads behave magnetically only in an external magnetic field, unlike ferromagnets. This property depends on the small size of the particles in the beads and allows the beads to be separated in suspension from whatever they are bound to. They can be used without fear of clumping because they do not attract each other outside a magnetic field.

What are the types of magnetic beads ?

Magnetic beads come in a variety of sizes and shapes. Each type of bead has binding properties that magnetically separates nucleic acids, proteins, and other biomolecules. This ease of use makes them automation-friendly and suitable for many applications, including sample preparation for next generation sequencing (NGS), protein purification, molecular and immunodiagnostics, and magnetically activated cell sorting (MACS).

What kind of purifiaction with the magnetic beads ?

Ultracoat50 coating on the magnetic beads enables the purification of :

  • The Biotinylated antibodies and other proteins
  • Biotinylated dsDNA (gDNA, PCR products) or biotinylated RNA
  • Biotinylated oligonucleotides

Contact Us if you want to learn more about our product or have any queries. e-Proteins guarantees the best prices for the highest quality.

UltraCoat50 Price

ProductQuantityPrice Dimerorder
UltraCoat (UCA)
100 mg1 310 €
UltraCoat (UCA)1 gr10 245 €
UltraCoat (UCA)10 gr92 736 €
UltraCoat (UCnA)100 mg1572 €
UltraCoat (UCnA)1 gr12 290 €
UltraCoat (UCnA)10 gr111 283 €

UltraCoat50 specifications

PhysicochemistryUltracoatUltracoat (neutral)
Purity100%100%
Activity
(U/mg solid)
> 14> 13
Moisture content
(% w :w)
< 5.0 %< 5.0 %
Extintion coefficient1.80-2.001.80-2.00
Isoelectric point9,5 ± 0,26,2 ± 0,2
Deglycosylation level± 90%± 90%
Dimer > 10%OKOK
Optimum Solubility
(transmittance @650nm
15mg/ml in water pH)
pH < 7 and pH > 10 pH < 5 and pH > 8

Details:

MicrobiologyUCA & UCnA
Total Bacterial Count in 1 mg< 1
OtherUCA & UCnA
Definition of
activity
One Unit activity binds

1 µg of biotin
Country of
Manufacturing
Belgium
OriginEgg proteins from hens
ApparenceWhite to off white free
flowing lyophilised
powder, free of any
visible impurities

UltraCoat50 CoA

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