Biotin blocking System

How to block non specific biotin ?

Adding BBS to the cell culture irreversibly binds free biotin, preventing it from breaking the avidinbiotinylated receptor complexes. This allows for a 1-column 3 steps affinity purification process on agarose-avidin chromatography columns.

Step 0

Introduced in slight excess to the cell culture (5-10%), the Biotin Blocking System irreversibly binds free biotin. A standard HABA test can be performed to verify the results.

Step 1

The avidin-agarose chromatography column is activated with the ligandspecific biotinylated receptors (antibody-specific biotinylated antigens for example).

Step 2

The Biotin blocking System saturated culture medium is passed through and rinsed with an adapted running buffer, leaving the specific ligand-receptors immobilized. no interaction between the biotin from the cell culture with the avidin-agarose chromatography column. All ligand-specific biotinlated receptor remains in place for the binding with the ligand of interest.

Step 3

The ligand of interest is then eluted out of the affinity column with the appropriate releasing buffer.

1-column 3 steps process for cell culture media purification. Biotin Blocking System serves as safeguard for column integrity and performance.

Prices

ProductPrices Order
BBS 500 ml390 €
BBS 10 x 500 ml3700 €

Specifications

PhysicochemistryBBS
Activity

(U/ml)
> 70
Avidin

(g/l)
5.5-6.0
Dry Matter 
%
(w:w)
< 11.0
Salts (KCl/NaCl)
%
(w:w)
< 5.0
Sodium Azide
%
(w:w)
0.02
Transmittance
OD
(650nm)
< 0.1
ColourYellow-reddish
Shelf life
Years
3
MicrobiologyBBS
Total Bacterial count
in 1 mg
<1
OtherBBS
Definition of
activity
One Unit activity binds

1 µg of biotin
Country of
Manufacturing
Belgium
OriginEgg proteins from hens

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