Biotin blocking System
How to block non specific biotin ?
Adding BBS to the cell culture irreversibly binds free biotin, preventing it from breaking the avidinbiotinylated receptor complexes. This allows for a 1-column 3 steps affinity purification process on agarose-avidin chromatography columns.
Introduced in slight excess to the cell culture (5-10%), the Biotin Blocking System irreversibly binds free biotin. A standard HABA test can be performed to verify the results.
The avidin-agarose chromatography column is activated with the ligandspecific biotinylated receptors (antibody-specific biotinylated antigens for example).
The Biotin blocking System saturated culture medium is passed through and rinsed with an adapted running buffer, leaving the specific ligand-receptors immobilized. no interaction between the biotin from the cell culture with the avidin-agarose chromatography column. All ligand-specific biotinlated receptor remains in place for the binding with the ligand of interest.
The ligand of interest is then eluted out of the affinity column with the appropriate releasing buffer.
1-column 3 steps process for cell culture media purification. Biotin Blocking System serves as safeguard for column integrity and performance.
|Total Bacterial count|
in 1 mg
|Definition of |
|One Unit activity binds
1 µg of biotin
|Country of |
|Origin||Egg proteins from hens|