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R&D program

Monodisperse HRP Conjugate

Optimized Ratio of HRP to Biotin-Binding Protein

Conjugate over current market offerings. Firstly, it uniquely enables direct quantification of biotinylated elements. Secondly, we have engineered eQuant-HRP to achieve an optimal balance between Horseradish Peroxidase (HRP) and biotin-binding protein. Thirdly, this carefully calibrated balance significantly enhances specificity in various assays, leading to more accurate and reliable detection.

Defined Composition

E-Proteins has been at the forefront of enhancing the quality of avidin products. From 2012 to 2014, significant investments in equipment and new technologies for avidin production led to achieving over 99% purity and more than 99.5% activity. This R&D initiative involved numerous investigations, some of which improved existing products, while others laid the groundwork for innovative derivatives.

Significant Reduction in Non-Specific Binding

The improved HRP to biotin-binding protein ratio in eQuant-HRP significantly reduces non-specific binding. This reduction not only increases the accuracy of assay results but also minimizes potential interference, providing clearer and more definitive outcomes.

Defined Composition

Industrial Standard

Crude mixture of huge agglomerates > 700kDa

HookDin features a unique polymeric structure that maximizes performance on nano gold particles: Structural Characteristics.
Homogeneous Polymerization: Gel filtration profile showing controlled oligomerization with predominant dimers, trimers, and tetramers

Covalent-Only Binding: Linker-free architecture ensuring optimal stability between molecules
Molecular Weight < 500 kDa: Constant extinction coefficient for perfect reproducibility
Multi-Layer Functionality: Extended binding surface compared to monomeric Neutralite Avidin

Higher Sensivity
for Precise Quantification

e-Proteins controls the production of specific avidin and HRP isoforms exhibiting pIs from acidic to basic pH.

Defined combinations in different conjugates enable controlled and directed manipulation of non-specific binding characteristics
thereby offering a controlled means for minimizing background reactions
in a given assay in immunohistochemistry (IHC), Western Blot, ELISA or ELISpot.

New development project using Phycoerythin (PE)

The e-Proteins’ conjugate generation offers an higher sensibility and it enables a direct quantification. Using the same components as the current conjugate available on the market, there is no need of revalidation.

The difference between both conjugates is only the method of conjugaison.

1:1 Neutralite Avidin: PE allowinfa direct quantification and les NSB

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