Eliminating Free Biotin Interference for Accurate Diagnostic Results
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High Biotin- Binding Capacity
How to block non specific biotin ?
Adding BBS to the cell culture irreversibly binds free biotin, preventing it from breaking the avidin biotinylated receptor complexes. This allows for a 1-column 3 steps affinity purification process on agarose-avidin chromatography columns.
1-column 3 steps process for cell culture media purification. Biotin Blocking System serves as safeguard for column integrity and performance.
Step 0
Introduced in slight excess to the cell culture (5-10%), the Biotin Blocking System irreversibly binds free biotin. A standard HABA test can easily verify the results.
Step 1
The avidin-agarose chromatography column is activated with the ligandspecific biotinylated receptors (antibody-specific biotinylated antigens for example).
Step 2
The Biotin blocking System saturated culture medium is passed through and rinsed with an adapted running buffer, leaving the specific ligand-receptors immobilized. no interaction between the biotin from the cell culture with the avidin-agarose chromatography column. All ligand-specific biotinlated receptor remains in place for the binding with the ligand of interest.
Step 3
The ligand of interest is then eluted out of the affinity column with the appropriate releasing buffer.
HookDin
Engineered to deliver up to three times the biotin binding capacity, HookDin ensures superior sensitivity and cleaner signals in lateral flow assays and gold particle applications.
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Neutralite Avidin
A deglycosylated avidin with high solubility and minimal non-specific binding, Neutralite Avidin provides a reliable and versatile alternative to streptavidin across multiple assays.
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Avidin
An innovative avidin derivative designed for advanced biotechnological applications, NAX combines optimized binding performance with the flexibility needed for custom research solutions.
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