How HookDin Triples Biotin Binding Capacity for Ultra-Sensitive Diagnostic Tests

Revolutionary Performance on Nano Gold Particles

HookDin demonstrates exceptional performance when conjugated on nano gold particles (NGP), delivering triple biotin binding capacity compared to conventional streptavidin. This biotechnology breakthrough revolutionizes diagnostic test sensitivity and reliability.

Proven Results

Comparative testing on Lateral Flow Assay (LFA) strips reveals spectacular outcomes:
3x Higher Signal Intensity: Nano gold particles coupled with HookDin generate detection signals three times stronger than standard streptavidinDelayed Hook Effect: NGP-HookDin technology successfully delays the Hook effect, extending the linear detection range
Enhanced Test Reliability: Significant reduction in false negatives through increased binding capacity

Multi-Layer Molecular Architecture

HookDin features a unique polymeric structure that maximizes performance on nano gold particles:
Structural Characteristics
Homogeneous Polymerization: Gel filtration profile showing controlled oligomerization with predominant dimers, trimers, and tetramers

Covalent-Only Binding: Linker-free architecture ensuring optimal stability between molecules
Molecular Weight < 500 kDa: Constant extinction coefficient for perfect reproducibility
Multi-Layer Functionality: Extended binding surface compared to monomeric Neutralite Avidin

Nano Gold Particle Advantages with Hookdin

Optimized Conjugation: Compatible with wide pH range for NGP conjugation
Triple Binding Capacity: Reduces bead saturation, minimizing diagnostic errors
Prevents Treatment Delays: Enhanced test reliability avoiding false diagnoses

Lateral Flow Assays (LFA)

HookDin transforms rapid test strip performance by offering unmatched sensitivity through its tripled binding capacity on nano gold particles.

e-Proteins Technological Innovation

Developed after 9 years of research and over 370 experiments , HookDin addresses the growing demand for higher binding signals for detection. This polymeric protein represents the natural evolution of Neutralite Avidin, combining:

1. Nearly neutral charge reducing background noise by 75%
2. No oligomannosidic glycans
3. RYD-free amino acid sequence (Arg-Tyr-Asp)
4. Controlled polymerization capability
5. Technical Specifications
6. Formulation: Lyophilized powder reconstitutable in any buffer
7. Filtration: 0.22-micron compatible
8. Stability: Resistant to temperature, pH, ionic strength variations
9. Flexibility: Available lysines for additional conjugations and derivatizations
10. High-Performance Direct Substitution

HookDin represents the best alternative to any cross-linking streptavidin technology , offering enhanced properties for next-generation in vitro diagnostics (IVD).

Key Benefits for Nano Gold Applications

1. Superior Surface Area: Enhanced biotin capture performance on NGP
2. Homogeneous Oligomerization: Consistent molecular weight distribution
3. Wide pH Compatibility: Suitable for various conjugation conditions
4. Minimized False Negatives: Triple binding capacity prevents test failures

Why Choose HookDin for Nano Gold Particles?

1. Proven Performance: Clinical data shows more than 200% signal intensity boost compared to conventional alternatives
2. Reliability: Homogeneous structure with constant extinction coefficient ensures reproducible results
3. Versatility: Compatible with LFA
4. Innovation: Covalent-only binding without linkers provides superior stability