Avidin Purification Systems for Biotechnology

Step 0

Introduced in slight excess to the cell culture (5-10%), the Biotin Blocking System irreversibly binds free biotin.

A standard HABA test can easily verify the results.

Step 1

The avidin-agarose chromatography column is activated with the ligandspecific biotinylated receptors (antibody-specific biotinylated antigens for example).

Step 2

The Biotin blocking System saturated culture medium is passed through and rinsed with an adapted running buffer, leaving the specific ligand-receptors immobilized. no interaction between the biotin from the cell culture with the avidin-agarose chromatography column. All ligand-specific biotinlated receptor remains in place for the binding with the ligand of interest.

Step 3

The ligand of interest is then eluted out of the affinity column with the appropriate releasing buffer.