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Avidin Lateral Flow Applications for Diagnostics

Introduction

e-Proteins provides avidin lateral flow solutions that improve assay sensitivity and signal clarity. HookDin technology delivers superior performance for point-of-care diagnostics.

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HookDin enables efficient surface functionalization to boost biotin-binding capacity. On gold nanoparticles (NGP) for lateral flow assays (LFA), HookDin delivered a 3× higher biotin-binding capacity versus monomeric streptavidin, resulting in higher signal sensitivity and a threefold delay of the high-dose hook effect.

HookDin—featuring the lowest known non-specific binding in its class—is tailor-made for spray deposition on LFA test lines. On nitrocellulose membranes it provides ~3× the biotin-binding capacity of monomeric streptavidin, delivering clear T-line signals, greater sensitivity, and a broader dynamic range—ideal for multiplex and quantitative LFAs, especially with small biotinylated targets (DNA, aptamers, nanobodies).

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