Poly-Lite Avidin

How Poly-Lite Avidin Ensures A Higher Binding Signal for Detection

This innovative product presents a higher binding signal due to its multi-layers forms. The higher binding capacity improves the test sensibility for accurate detection. It is not only the best alternative to cross-linking streptavidin technology or “polystreptavidin R” but also has improved properties. Let’s learn the distinct characteristics of Poly-Lite Avidin.

Poly-Lite Avidin Characteristics

  • It is homogeneous with a constant extinction coefficient and molecular weight < 500 kDa as compared to the conventional polystreptavidin R.
  • The product has only covalent binding between the molecule without linker.
  • Its conformation stays simple because of covalently linked molecules.
  • Temperature, pH, protein concentration, ionic strength, as well as molecular crowding have a limited impact on the olimegorized protein.
  • The Smooth polymerization process enables the use of Lysine (available for further conjugation and derivatization).
  • e-Proteins product Poly-Lite Avidin can be used in any range of pH.
  • Have a higher level of dimers, trimers and quadrimer comparing the cross-linking steptavidin profile.

e-Proteins developed two chemical modifications to keep the product soluble for use in acid/neutral reconstitution buffer as well as neutral/basic reconstitution buffer, a novel approach to high-performance in vitro diagnostics (IVDs) and direct substitution. This meets the specific needs of the customer regarding the reconstitution buffer.

 

Poly-Lite Avidin Development Background

In 2012, Customers who use the cross-linking streptavidin technology requested e-Proteins to develop a new product that merges the benefits of Neutralite Avidin with the high sensitivity of cross-linking streptavidin. In one word, the customer looks for a new derivative that offer a higher binding signal for detection.

After eight years of research, e-Proteins developed a process to produce Poly-Neutralite Avidin with a profile that has more dimers, trimers, and quadrimers than cross-linking Steptavidin R.

Neutralite Avidin, with a nearly neutral charge, no oligomannosidic glycan as well as no Arg-Tyr-Asp (RYD) amino acids sequence, has reduced background or non-specific bio-recognition by 75% for years.

Poly-Lite Avidin from e-Proteins boosts signal intensity by 130%, improving specificity by at least 500% over Avidin. Today this derivative presents the higher binding signal for detection on the market.

 

High purification rate biotin binding.

 

If you are interested in our product and have any questions, please do not hesitate to Get in Touch with us. At e-Proteins, you can buy with confidence, knowing that you will get the lowest prices on the best products.

Prices

ProductQuantityPrice trimerorder
Poly Lite Avidin (PLA)
100 mg2 820 €
Poly Lite Avidin (PLA)1 gr19 800 €
Poly Lite Avidin (PLA)10 gr158 800 €
neutral Poly Lite Avidin (nPLA)
100 mg3 170 €
neutral Poly Lite Avidin (nPLA)
1 gr21 870 €
neutral Poly Lite Avidin (nPLA)
10 gr175 590 €

Specifications

PhysicochemistryPoly Lite AvidinPoly(neutral)Lite Avidin
Purity100%100%
Activity
(U/mg solid)
> 14> 13
Moisture content
(% w :w)
< 5.0 %< 5.0 %
Extintion coefficient1.80-2.001.80-2.00
Isoelectric point9,5 ± 0,26,2 ± 0,2
Deglycosylation level± 90%± 90%
trimer > 3%OKOK
Optimum Solubility
(transmittance @650nm
15mg/ml in water pH)
pH < 7 and pH > 10 pH < 5 and pH > 8
MicrobiologyPoly (n)Lite avidin
Total Bacterial Count in 1 mg< 1

Details:

OtherPoly (n)Lite Avidin
Definition of
activity
One Unit activity binds

1 µg of biotin
Country of
Manufacturing
Belgium
OriginEgg proteins from hens
ApparenceWhite to off white free
flowing lyophilised
powder, free of any
visible impurities

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