Products Comparison

Avidin & Neutralite Avidin bind four biotins per molecule whereas Nitrolite Avidin binds three of them, with high affinity and selectivity. Their multiple binding sites permit a number of bridging techniques using biotinylated reagents. This bridging method is commonly used to link a biotinylated probe to a biotinylated enzyme in enzyme-linked immunohistochemical applications. It often eliminates the background problems that can occur when using direct avidin–enzyme conjugates. read more



    Native Avidin Neutralite Avidin Nitrolite Avidin Streptavidin  
     
    Biotin Irreversible Reversible at pH 10    
    Iminobiotin Reversible at pH 4 Reversible    
Physicochemistry Batch N° AVI NLA NTA STREP-T7 Note
Activity U/mg solid > 14,0 > 14,0 > 10,0 > 14,0 P1
ID Test HABA Test OK OK OK OK P2
Moisure % (w:w) max.) 5,0 5,0 5,0 5,0 P3
Protein Purity SDS Page at 10 mg No unspecified band No unspecified band No unspecified band No unspecified band P4
Molecular Weight kDa 66 60 60 60 P5
Isoelectric Point +/- 0,5 10,0 6,5 6,5   P6
Product Life Years 3 3 3 3 P7
Microbiology            
Total Viable Count TVC/gm < 10 < 10 < 10 < 10 B1
LPS Endotoxins IU/mg N/A N/A N/A N/A B2
VVND - absent absent absent absent B3
Enzymology            
DNA-ase Endo/exonuclease free free free free E1
RNA-ase Ribonuclease free free free free E2